BioFlux Applications
Cellular Analysis, Research, and Imaging

Many physiological processes take place under flow conditions: blood flowing through the vasculature; cancer cells circulating throughout the body; plaque forming on teeth under the presence of saliva flow. It is now well established that physiological flow has a profound impact on many biological studies, yet much research is still conducted in vitro without the presence of flow.
BioFlux from Fluxion Biosciences gives you the ability to introduce flow to your research and drug discovery experiments, effectively emulating in vivo conditions and revealing the true biology.
Check the application pages to see how BioFlux can accelerate your live cell assays.
“Given the critical role of shear in regulating platelet adhesion and thrombus growth, these findings may have potential pathophysiological significance.” [Jackson, et al., 2006]
Hematology Applications
Thrombosis, Platelet Activity, and Atherosclerosis Models
Immunology Applications
Immune Cell Analysis, Cell Adhesion/Rolling, and Transmigration
Oncology Research Applications
Engineered T cells, CTCs, and Tumor Microenvironment
Oral Biofilms
Use saliva as media to replicate oral cavity for biofilm growth
Lung Biofilms
Anti-biofilm drug discovery for treatment of pulmonary diseases
Host-Pathogen Interactions
Form fungal and bacterial biofilms on epithelial monolayers in vitro
Bacterial Chemotaxis
Explore influence of genetic and morphological factors on surface motility
Mutant Screens
Use controlled shear flow on knockout mutant biofilms under physiologically relevant conditions
Medical Device Infections
PDMS and custom microfluidic plates mimic medical device infection environment
Antimicrobial Screens
Analyze effectiveness of novel antimicrobials on biofilm growth under shear flow
Adhesion Strength
Quantify biofilm adhesion and measure binding specificity in a microfluidic channel
COVID-19 Research
Study causes and treatments in COVID-19 patients
Migration & Invasion
Label-free, real-time measurements in situ
Atherosclerosis Models
Mimic arterial plaques under shear flow in vitro
Platelet Adhesion & Aggregation
Quantify platelet adhesion and aggregation area live
Thrombosis
Explore thrombus formation using whole blood
Sickle Cell Disease
Investigate adhesive properties of sickle erythrocytes in a simulated blood environment
Stem Cells
Analyze effectiveness of novel antimicrobials on biofilm growth under shear flow
Vascular Biology
Study vascular physiology, vascular cell-blood interactions, T-cell transmigration, wound healing, angiogenesis, mechanotransduction, and more!
Immunotherapy Development
Accelerate your engineered T cell drug discovery with functional testing of adhesion and transmigration in vitro
Cell Adhesion & Rolling
Utilize time-lapse microscopy under flow to quantify cell rolling and firm adhesion
Transmigration & Migration
Temperature-controlled transmigration experiments under shear flow
Rheumatology
Investigation of rheumatic disease under shear flow
Wound Healing
Generate more reproducible results than traditional scratch wound assays
Stem Cells
Investigate stem cell function under flow: homing, adhesion, transmigration, and differentiation
CAR-T/TCR Engineered T-Cell
Use saliva as media to replicate oral cavity for biofilm growth
Tumor Microenvironment
Control and manipulate the tumor microenvironment and test potential treatments
Metastasis
Examine metastasized cancer cells on endothelial monolayers and protein coatings
Cancer Cell Homing
Create 2 connected micro-environments to study cancer cell homing
Epithelial-Mesenchymal Transition
Analyze steps of cancer progression and EMT with cancer cells under shear flow
Cancer Cell Migration & Invasion
Create two adjacent micro-environments to analyze cell migration and invasion
Circulating Tumor Cells
Analyze CTC adhesion and clustering under shear flow
Leukemia
Examine leukocyte adhesion under shear flow conditions
Cancer Stem Cells
Analyze stem cell homing, adhesion, transmigration, and differentiation under flow
Customer Spotlight
Modeling of Catheter-Associated Infection
BioFlux was used in a recent study modeling catheter-associated infection by using BioFlux microfluidics to mimick the in vivo conditions of urinary catheters. In their lab, the BioFlux 200 system has been used to evaluate the efficacy of poloxamers in reducing E. coli adhesion under controllable shear forces by exploiting BioFlux silicone microfluidic flow channels, thus mimicking the in vivo conditions of urinary catheters in terms of both flow conditions and catheter material.
