Platelet-Inspired Intravenous Nanomedicine for Injury-Targeted Direct Delivery of Thrombin to Augment Hemostasis in Coagulopathies
Arterial thrombosis is a significant cause of myocardial infarction and ischemic stroke. Recent investigations have focused on abnormalities in the endothelium, platelets, and blood flow. Increased baseline platelet activity in response to mechanical and chemical stimuli leads to robust aggregation; therefore, investigation into methods of thrombosis prevention is paramount.
In this article by Liu et al., the group investigated the mechanisms of water-soluble tomato concentrate (WSTC) in the prevention of platelet thrombus formation both in vitro and in vivo. The BioFlux shear flow system was used to determine the impact of various WSTC concentrations on platelet adhesion and rolling speeds.
Visualization of cellular processes in real-time provides invaluable insight into cell biology. Cell adhesion through protein-protein or protein-carbohydrate interactions plays an important role indirecting cell migration, gene expression, cell growth, morphological changes during development, and programmed cell death.
The BioFlux system is particularly adaptable to platelet adhesion & aggregation screens and can be performed under physiologically-relevant shear flow conditions. With this system, platelet rolling, platelet adhesion, platelet aggregation, and adhesion inhibition can be quantitated under continuous flow, providing major work flow advantages over traditional adhesion assays.
Functionalized BioFlux flow channels were used to analyze the effect of guanosine on platelet adhesion and aggregation, mimicking arterial flow conditions. Guanosine was shown to inhibit platelet aggregation (Palomo et al., 2013).
Whole blood labeled with calcein AM flowing over collagen 1 at 40 dyn over 5 minutes.
Study live platelet adhesion and aggregation in a physiologically-relevant assay using primary endothelial cells, human peripheral blood mononuclear cells, and more
Investigation of kinetic platelet adhesion and aggregation behavior on different substrates over many parallel conditions
Utilize time-lapse microscopy under flow and post-experiment image analysis to determine rolling distance
Examine dozens of different monolayers at one time in order to determine IC50 concentrations or screen multiple compounds under the same experimental conditions
Calculate area of platelet adhesion by utilizing BioFlux software analysis capabilities