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Cell Microsystems Acquires Fluxion Biosciences to Broaden Cell Analysis Product and Services Portfolio. Read the Press Release

At Fluxion, we’re passionate about delivering cell-based solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

Wound Healing

and cell proliferation

Scratch wound assays are commonly performed to assess cellular migration and cell proliferation, often in response to therapeutic drug application. Wounding assays are also used to further understand the molecular phenomena related to wound healing, which includes cell signaling, immune and healing response to bacterial and fungal infections, and tissue remodeling. Most wounding assays are performed using a mechanical means to generate a break in a confluent monolayer of cells. Often this involves drawing a pipet tip or a pin over the monolayer. This physically damages the monolayer as desired, but also damages the cells, causing contents to leak and potentially complicating the interpretation of data. The other drawback of this method is that wound size and shape is highly variable contributing to difficultly in data analysis and inability to directly compare experimental conditions.

The BioFlux system can be used to wound cells enzymatically. The microfluidic channels in the BioFlux plate are identical within <5%, conferring the ability to generate wounds by fluid flow that are highly regular in shape and similar in size. Migration and proliferation following wounding can be analyzed by microscopy using promoters of cell migrations, epidermal growth factors, and motility and cell cycle inhibitors. 

FEATURED ARTICLE

Fc-modified HIT-like monoclonal antibody as a novel treatment for sepsis

Sepsis leads to multiorgan dysfunction and is associated with increased morbidity and mortality, worldwide. During sepsis, neutrophils release extracellular traps (NETs) to capture pathogens, however, NETs have also been found to contribute to tissue damage.

In this featured article, Gollomp et al. examined potential therapeutics aimed at reducing sepsis-related tissue damage induced by NETs. To accomplish their goal, the authors developed and deployed recombinant immunoglobulin G2bk anti-hPF4/heparin monoclonal antibody (hPF4). Human umbilical vein endothelial cells (HUVECs) were cultured and activated in the BioFlux system, allowing the investigators to examine the impact of hPF4 treatment on NET entrapment.

Read the article

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Wound healing assay in BioFlux 24-well plate

This is a wound healing assay performed using the 24 well dual flow plates. After a monolayer of HUVEC cells is seeded, the growth media remains in inlet A (top) while trypsin is introduced in inlet B (bottom) removing half of the cells. Growth media is then introduced and the rate of cell proliferation is measured.

HubSpot Video

 

Key BioFlux Advantages For Wound Healing Studies

  • Achieve a wounding effect by using the two inlet one outlet well plate and trypsinizing half of the channel

  • Quantify wound size, cell migration, and cell proliferation

  • Generate tightly controlled wounds by enzymatic means and fluid flow

  • Cells at the leading edge of the wound remain intact; thus cytosolic leakage is not a factor in the interpretation of the data

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