Studying Fungus-Bacterium Interactions Under Dynamic Conditions
Dr. Bastiaan P. Krom Ph.D.
Associate Professor of Preventive Dentistry
Department of Preventive Dentistry,
Academic Centre for Dentistry Amsterdam (ACTA)
University of Amsterdam
Free University Amsterdam
The fungus Candida albicans is a commensal to most, if not all, mammals. This versatile fungus thrives in diverse niches of humans such as the skin, GI-tract, vagina and the oral cavity. In all of these niches it encounters different bacteria. It is therefore not surprising that C. albicans has developed specific systems to interact with co-inhabiting bacteria. These interactions can be chemical (signaling and metabolic) or physical (adhesion and aggregation) in nature. In this webinar I will present approaches we currently use to study dynamics of these interactions and their biological function. In particular I will focus on real-time analysis of bacterial adhesion to C. albicans and on the effect of fungus-bacterium interactions in relation to macrophage function. In addition, I will discuss how we study the effect of bacterial small molecules on fungal behavior.