BioFlux 200 User's Guide

Table of Contents

  1. Safety and Site Requirements
    1. Safety considerations
    2. Power requirements
    3. Bench space
    4. Environmental considerations
  2. Instrument Installation
    1. Unpacking and inspecting the system
    2. Setting up pneumatic connections
    3. Setting up electrical connections
    4. External Gas Supply connections
  3. Software Setup and Functionality
    1. Installing and loading the software module
    2. Hardware Connections
    3. Fluid Properties
    4. Manual Mode
    5. Edit AutoRun
    6. AutoRun Mode
    7. Image Mode
    8. Analysis Mode
  4. Running Experiments
    1. Cell and reagent preparation
    2. Film-punching template for use with plate sealing film
    3. Connecting the Interface
    4. Setting up temperature control
    5. Priming the channels
    6. Coating the channels
    7. Introducing cells and creating cellular monolayers
    8. Establishing shear flow
    9. BioFlux Plate management and refilling
  5. Troubleshooting Guide
    1. Troubleshooting hints
    2. Technical Support Contacts
  6. Warranty Information
  7. Tips and Tricks

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