Cellular Adhesion: Adhesion of colon carcinoma cells and leukocytes to endothelial cells

Endothelial cell adhesion is the first step in a number of processes in human health and disease. In normal functioning of the immune system, white blood cells are recruited through the vascular endothelium to the underlying tissue to perform tasks related to inflammation and injury. In metastatic disease tumor cells can invade tissues using similar mechanisms of transmigration as immune cells. One of the first steps in either process is cell adhesion to the endothelial cells lining the blood vessels.

Using the Bioflux 200 system (Figure 1), a multiplexed, microscale, shear flow system optimized for tight shear control and capture of microscopy data, endothelial cell monolayers were grown to full confluence and activated with IL-1β. Leukocyte and colon carcinoma cells were introduced onto the endothelial cell monolayers under flow and adhesion data were captured using microscopy.