All silicone plate format
Our aim was to provide a primary attachment and growth surface with silicone chemistry (PDMS) to mimic catheter and other silicone medical device surfaces. For BioFlux plates currently in use, the primary growth surface is a glass (borosilicate) coverslip, 170μm thick. This provides excellent compatibility with all microscopy imaging modalities. In order to convert to silicone, a PDMS layer is molded against highly polish surfaces to create a flat substrate that is 250um thick across the full device bottom and secures the channels (Figure 3). The increased thickness is necessary in order to maintain mechanical stability and is within the compatibility zone for commonly used objectives. The resulting all-silicone flow channels are ideal for studying biofilm formation on medical devices such as indwelling catheters.