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Cell Microsystems Acquires Fluxion Biosciences to Broaden Cell Analysis Product and Services Portfolio. Read the Press Release

At Fluxion, we’re passionate about delivering cell-based solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

Antimicrobial Screening

and biofilm treatment

The BioFlux system is a microfluidic platform designed to run automated shear flow protocols microbiology experiments. A 48-well BioFlux plate can run up to 24 experiments simultaneously. To increase throughput even further, the BioFlux Quattro system operates up to 96 simultaneous experiments, making it ideal for antimicrobial screening assays on biofilms. 

In the paper below, various compounds were tested to determine their ability to prevent or eradicate biofilms. 

s mutans

(A) S. mutans biofilms were cultured in a BioFlux system for 18 to 24 hours, inoculated with different antibacterial agents, and stained (live cells stained green and dead cells stained red). (B) These results were graphed, showing statistically significant differences between the control and the antimicrobial-treated groups (Ding et al., 2014).

antimicrobial screening s mutans

S. mutans was grown on a BioFlux plate, then treated with pleurocidin, and stained (green being the live cells and red being dead). Bacteria on the outside of the biofilm was killed by the antimicrobial agent, whereas the bacteria closer to the inside of the biofilm was protected by the surroundings (Tao et al., 2011).

Key BioFlux Advantages For Antimicrobial Screening Studies

  • Analyze the effectiveness of different antimicrobial compounds by growing biofilms under controlled shear flow, mimicking the conditions of natural growth

  • Use the all silicone plate format for antimicrobial compounds relating to catheter-associated infections

  • Custom substrate plates feature the ability to add any material to the bottom of microfluidic flow chambers, and enable a full range of experimental protocols for a variety of microbes and antimicrobial compounds

  • Discover new antimicrobial compounds and help eradicate antibiotic tolerance and susceptibility with a flow-based system


Examination of biofilms grown in microfluidic flow cell arrays for compound screening, host-pathogen interactions, and microbial adhesion

Higher-throughput biofilm assays often employ well plates for compound, mutant and conditional screening. Despite their throughput and convenience, well-plate assays have difficulty producing
higher content biofilm data, such as morphology, compound penetration, analysis of growth under flow, and analysis of mixed species interactions.

We present the BioFlux microfluidic flow cell array, which incorporates high content flow-based biofilm experiments integrated into a standard well-plate format.


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