(A) S. mutans biofilms were cultured in a BioFlux system for 18 to 24 hours, inoculated with different antibacterial agents, and stained (live cells stained green and dead cells stained red). (B) These results were graphed, showing statistically significant differences between the control and the antimicrobial-treated groups (Ding et al., 2014).
S. mutans was grown on a BioFlux plate, then treated with pleurocidin, and stained (green being the live cells and red being dead). Bacteria on the outside of the biofilm was killed by the antimicrobial agent, whereas the bacteria closer to the inside of the biofilm was protected by the surroundings (Tao et al., 2011).
FEATURED POSTER
Examination of biofilms grown in microfluidic flow cell arrays for compound screening, host-pathogen interactions, and microbial adhesion
Higher-throughput biofilm assays often employ well plates for compound, mutant and conditional screening. Despite their throughput and convenience, well-plate assays have difficulty producing
higher content biofilm data, such as morphology, compound penetration, analysis of growth under flow, and analysis of mixed species interactions.
We present the BioFlux microfluidic flow cell array, which incorporates high content flow-based biofilm experiments integrated into a standard well-plate format.
