Visualization of stem cell interactions under flow helps elucidate stem cell recruitment, differentiation, migration. Stem cells are increasingly studied as potential sources of therapeutic treatment, and the study of these processes under physiological conditions is critical.
The BioFlux system is particularly use for for stem cell studies under physiologically-relevant shear flow conditions. With this system, recruitment, cell adhesion, and transmigration can be quantitated under continuous flow, providing major work flow advantages over traditional flow cell assays.
Below is an example of a stem cell study using BioFlux
hPMVECs were seeded in BioFlux microfluidic channels to form monolayers and the application of 2 dyn/cm2 pressure allowed fluorescently labelled MSCs to pass over the monolayer under shear flow. Still images from recordings indicate little to no binding of MSCs (A) compared to Mo control cells under the same conditions (B). hPMVEC monolayers were treated with PBS (C, Panel ii) or LPS (C, Panel iii) before MSC suspensions were passed over the layers. Flow was stopped and the cells were allowed to settle on the monolayer for 30minutes (C, Panel i) before re-initiation of the flow. Once all cells had passed through the chamber, images were acquired of the remaining, firmly adherent cells (C, Panels i, ii). MSC: Mesenchymal Stem Cell; LPS: Lipopolysaccharide; Mo: Monocyte; hPMVEC: human pulmonary microvascular endothelial cells.
Key BioFlux Advantages For STEM Cell research
Accurately model the in vivo environment: study stem cell interaction and transmigration to cell layers such as HUVECs
Generate superior data: utilize time-lapse microscopy under flow and post-experiment image analysis to determine degree of adhesion and transmigration
Get results fast: examine dozens of different treatments to see the effect on stem cell recruitment
Easily analyze data: calculate cell numbers adhered/transmigrated by utilizing BioFlux software analysis capabilities